SARS-CoV-2 monitoring needs more than just antibodies...

Complete your view with T cells

T-SPOT® Discovery SARS-CoV-2: Proven T cell technology

The T-SPOT Discovery SARS-CoV-2 kit uses proven T cell technology to detect and measure the strength of the T cell response to SARS-CoV-2. We have over 17 years’ experience in developing leading T cell technology, and work in collaboration with government bodies including Public Health England and UK Vaccines Task Force.

T cells in COVID-19

Helen Fletcher, Professor of Immunology at the London School of Hygiene and Tropical Medicine, talks about the importance of T cells in COVID-19 and answers some topical questions, including: What role might T cells have in COVID-19? Are antibodies or T cells more important to COVID-19? How might T cells be used for testing?

Is there a role for T cells in the assessment of SARS-CoV-2 infection status?

Hear from Dr Ruth Brignall, Medical Science Liaison at Oxford Immunotec, discussing how T cell testing and diagnostics have been an incredibly important focus of the scientific community’s response to the COVID-19 pandemic including the role of T cells in the immune response to SARS-CoV-2 infection, the principles of cell-mediated immunity testing and the T-SPOT Discovery test for SARS-CoV-2 and its potential role in assessing infection status during the COVID-19 pandemic.

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Importance of T cell testing in SARS-CoV-2

Hear from Ruth Brignall, Medical Science Liaison at Oxford Immunotec, who recently presented at the World Vaccine Congress overviewing SARS-CoV-2 specific antibody decay and emerging evidence supporting T cell testing in the fight against COVID-19.

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Our T-SPOT 𝘋𝘪𝘴𝘤𝘰𝘷𝘦𝘳𝘺 SARS-CoV-2 kit

Hear from Rumel Ahmed, Director of Research and Development at Oxford Immunotec, as he explains the T-SPOT technology platform and how it has been used to create the new kit.

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Our expertise in T cells

Hear from Oxford Immunotec’s Augustina Gyamfiwah-Marfoh, Global Medical Affairs Operations Manager, and Yupei Xiao, Medical Science Liaison, as they share the company’s expertise in T cell measurement .

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100 Physicians Find: New testing needed to make the grade

Oxford Immunotec is pleased to welcome you to the first instalment of 100 Physicians Find, a monthly series in which we ask a hundred physicians on the front lines of the COVID-19 pandemic about their perspectives, experiences and needs in the face of this ongoing public health crisis.
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Why choose the T-SPOT technology?

The T-SPOT technology is unique as the only globally regulated ELISPOT platform, currently being used clinically for identifying and measuring T cells. The platform makes highly standardised measurement of the T cell response easy through centralisation of fresh blood samples from different study locations, as well as enabling automation leading to ease of use and scalability. These attributes are essential in large-scale studies, or for more widespread testing.

Tested technology

18 years’ experience of clinical T cell measurement using the T-SPOT technology

> 20M tests sold

Experienced manufacturer of IVD tests and approved in over 50 countries

Central processing

Extended sample stability (54hr) and automation for flexible sample processing

Optimal antigens

Investigate the full breath of the T cell immune response

Discovery

RESOURCE CENTRE

This is the central resource for all things T-SPOT Discovery SARS-CoV-2 related. Find out more about our leading T cell technology and further detailed information about how this technology can be applied to SARS-CoV-2.

Package insert: T-SPOT Discovery SARS-CoV-2 Webinar: The role of cell-mediated immunity in immune responses to SARS-CoV-2 infection White paper: T cell testing in SARS-CoV-2

How the T-SPOT technology works

Our T-SPOT test based on ELISPOT technology is normalised for both cell number and culture conditions. This means that the test standardises the number of cells and removes serum factors that could adversely affect results, making it the most sensitive and specific test for T cell measurement.

  1. A blood sample is collected using routine phlebotomy and a standard blood collection tube from which a subset of white blood cells, known as peripheral blood mononuclear cells (PBMCs), are isolated. The cells are washed, counted and normalised to create a standard cell suspension.
  2. A standard number of cells are added into specially designed plates and stimulated with antigens specific to the disease under study. Cells responding to these antigens release a chemical messenger known as a cytokine.
  3. Cytokine antibodies are used to directly capture the cytokine as it is released by the cells. A secondary labelled antibody is added and binds to the captured cytokine.
  4. A detection reagent is added and reacts with the secondary labelled antibody. This reaction produces spots, which are a footprint of where the cytokine was released. Spots are then enumerated.